Gram Stain Set, 4x4oz, Microscope slides, smears, bacteria

Description

Gram Stain Set, 4oz, Microscope slides, smears
This Gram Stain Set consists of four chemicals, each in a handy 4oz plastic dropper top bottle.
This set contains...
* SOLUTION #1:
CRYSTAL VIOLET 0.5% with 10 % Ethanol and 0.8% Ammonium Oxalate.
* SOLUTION #2:
GRAM’S IODINE, PVP-iodine complex (PVP-iodine is a stable chemical  complex of  polyvinylpyrrolidone (PVP) and elemental iodine which is completely soluble in water, ethanol etc.).
* SOLUTION #3:
ACETONE/ALCOHOL DECOLORIZER 1:1 Acetone and Ethanol
* SOLUTION #4:
SAFRANIN 0.5%
Procedural Overview...
Danish physician, Hans Christian Gram, published his stain procedure in 1884, which he had developed to enable bacteria to be seen more readily in stained sections of lung tissue.
In current practice, the procedure is used in bacterial identification to differentiate between 2 classes of bacteria, Gram-positive and Gram-negative.  Differentiation of the two types of bacteria requires four solutions used in succession on a properly prepared slide sample of bacteria.
Important Preliminary Process . . .
Prior to the staining process, the slide/smear requires “proper” preparation as follows:
a.  Make a THIN film of the material on a clean glass slide, using sterile swab/loop for viscous specimens.
b.  AIR DRY and then HEAT FIX slide, passing several times through flame (should not become too hot to touch.)
If not properly FIXED, smears may be washed away resulting in absence of stained bacteria.
Slide/Smear Stain Preparation. . .
1.  Flood slide with Solution #1 —
CRYSTAL VIOLET— for 10 seconds and then wash with running tap water.
2.  Flood slide with Solution #2 -
GRAM’S IODINE — for 10 seconds, wash off excess iodine solution with running tap water.
3.  Wash sample quickly and carefully with Solution #3 - DECOLORIZER
- this third step is critical as applying the decolorizer for too long will result in washing away the purple iodine-dye complexes
in both the Gram-positive and negative bacteria.
4.  Flood the slide  with Solution #4 -
SAFRANIN
-- for 10 seconds to counterstain and then wash with water.
Process Recap. . .
After all 4 steps of the Gram staining process are completed, bacterial samples which appear blueish-purple are termed Gram-positive and those which are stained red from the Safranin are termed Gram-negative.
Caution: This chemical kit may only ship to destinations within the 48 contiguous US states and Canada using ground ORMD shipping only.